November 19-22, 2003

Ljubljana, Slovenia

Electroporation based Technologies and Treatments

International SCIENTIFIC WORKSHOP and POSTGRADUATE COURSE

Key lectures and content of the course:

Cells and tissues in electric fields

D. Miklavèiè
  • Biological cell and its plasma membrane
    		•
  • Resting and induced transmembrane voltage
    		•
  • Static and dynamic electric fields in biological tissues
    		•

    Electroporation, part 1

    E. Neumann
  • The history of electroporation
    		•
  • Elementary theory of electroporation
    		•
  • Electroporation of lipid bilayers
    		•
  • Electroporation of vesicles
    		•

    Electroporation, part 2

    J. Teissié
  • Electroporation of cells in suspension
    		•
  • Electroporation of cells in 2D tissue model
    		•
  • Parameters that determine the efficiency of electroporation in vitro
    		•

    Electroporation, part 3

    L.M. Mir
  • Electroporation of cells in tissue
    		•
  • Methods for detecting cell electropermeabilization in vivo
    		•

    Development of devices and electrodes

    D. Miklavèiè
  • Devices for in vitro and in vivo experimental work
    		•
  • Devices for clinical purposes
    		•
  • Electrodes for in vitro and in vivo
    		•

    Application of electroporation in electrochemotherapy of tumors

    G. Ser¹a
  • Experiments in vitro
    		•
  • Experiments in vivo
    		•
  • Current clinical applications
    		•
  • Future perspectives
    		•

    Application of electroporation in gene transfection

    L.M. Mir,
  • Gene transfection in vitro
    		•

    J. Teissié
  • Gene transfection in vivo
    		•
  • Future perspectives
    		•

    Application of electroporation in biotechnology

    J. Teissié
  • Water sterilization
    		•
  • Other applications
    		•
  • Future perspectives
    		•

    Electroporation in transdermal drug delivery

    V. Preat
  • Transdermal drug delivery using electroporation
    		•
  • Intradermal and topical delivery of DNA by means of electroporation
    		•

    Short presentations of selected abstracts

    Round table on electroporation based technologies and treatments